Luaine Bandounas, Martijn Pinkse, Johannes H de Winde, Harald J Ruijssenaars
Publication date
Journal name
New Biotechnology
In this study we have investigated the molecular background of the previously reported dye
decolourization potential of Bacillus sp. LD003. Strain LD003 was previously isolated on
Kraft lignin and was able to decolourize various lignin model dyes. Specifically Azure B (AB)
was decolourized efficiently. Proteins possibly involved in AB decolourization were partially
purified, fractionated by gel electrophoresis and identified via mass spectrometry. Five
candidate enzymes were selected and expressed in Escherichia coli. Of these, only a ...
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