Title
Authors
Stefan de Kok, Duygu Yilmaz, Jean-Marc Daran, Jack T Pronk, Antonius JA van Maris
Publication date
2012/8/1
Journal name
Antonie van Leeuwenhoek
Volume
102
Issue
2
Pages
401-406
Publisher
Springer Netherlands
Description
Abstract Plasma membrane H+-ATPase isoforms with increased H+/ATP ratios represent a
desirable asset in yeast metabolic engineering. In vivo proton coupling of two previously
reported Pma1p isoforms (Ser800Ala, Glu803Gln) with increased in vitro H+/ATP
stoichiometries was analysed by measuring biomass yields of anaerobic maltose-limited
chemostat cultures expressing only the different PMA1 alleles. In vivo H+/ATP
stoichiometries of wildtype Pma1p and the two isoforms did not differ significantly.
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