Simultaneous single-molecule measurements of phage T7 replisome composition and function reveal the mechanism of polymerase exchange JJ Loparo, AW Kulczyk, CC Richardson, AM Van Oijen Proceedings of the National Academy of Sciences 108 (9), 3584-3589, 2011 | 130 | 2011 |
Simultaneous real-time imaging of leading and lagging strand synthesis reveals the coordination dynamics of single replisomes KE Duderstadt, HJ Geertsema, SA Stratmann, CM Punter, AW Kulczyk, ... Molecular cell 64 (6), 1035-1047, 2016 | 73 | 2016 |
Single-molecule studies of polymerase dynamics and stoichiometry at the bacteriophage T7 replication machinery HJ Geertsema, AW Kulczyk, CC Richardson, AM Van Oijen Proceedings of the National Academy of Sciences 111 (11), 4073-4078, 2014 | 73 | 2014 |
Structural and functional probing of PorZ, an essential bacterial surface component of the type-IX secretion system of human oral-microbiomic Porphyromonas gingivalis. AM Lasica, T Goulas, D Mizgalska, X Zhou, I De Diego, M Ksiazek, ... Scientific Reports 6 (1), 37708, 2016 | 67 | 2016 |
Conformational dynamics of bacteriophage T7 DNA polymerase and its processivity factor, Escherichia coli thioredoxin B Akabayov, SR Akabayov, SJ Lee, S Tabor, AW Kulczyk, CC Richardson Proceedings of the National Academy of Sciences 107 (34), 15033-15038, 2010 | 55 | 2010 |
The replication system of bacteriophage T7 AW Kulczyk, CC Richardson The Enzymes 39, 89-136, 2016 | 36 | 2016 |
Solution structure and DNA binding of the zinc-finger domain from DNA ligase IIIα AW Kulczyk, JC Yang, D Neuhaus Journal of molecular biology 341 (3), 723-738, 2004 | 36 | 2004 |
Cryo-EM structure of the replisome reveals multiple interactions coordinating DNA synthesis AW Kulczyk, A Moeller, P Meyer, P Sliz, CC Richardson Proceedings of the National Academy of Sciences 114 (10), E1848-E1856, 2017 | 30 | 2017 |
Coupling dTTP hydrolysis with DNA unwinding by the DNA helicase of bacteriophage T7 AK Satapathy, AW Kulczyk, S Ghosh, AM Van Oijen, CC Richardson Journal of Biological Chemistry 286 (39), 34468-34478, 2011 | 30 | 2011 |
An interaction between DNA polymerase and helicase is essential for the high processivity of the bacteriophage T7 replisome AW Kulczyk, B Akabayov, SJ Lee, M Bostina, SA Berkowitz, ... Journal of Biological Chemistry 287 (46), 39050-39060, 2012 | 29 | 2012 |
Direct observation of enzymes replicating DNA using a single-molecule DNA stretching assay AW Kulczyk, NA Tanner, JJ Loparo, CC Richardson, AM van Oijen JoVE (Journal of Visualized Experiments), e1689, 2010 | 21 | 2010 |
Inadequate inhibition of host RNA polymerase restricts T7 bacteriophage growth on hosts overexpressing udk U Qimron, AW Kulczyk, SM Hamdan, S Tabor, CC Richardson Molecular microbiology 67 (2), 448-457, 2008 | 20 | 2008 |
Electron microscopy holdings of the Protein Data Bank: the impact of the resolution revolution, new validation tools, and implications for the future SK Burley, HM Berman, W Chiu, W Dai, JW Flatt, BP Hudson, JT Kaelber, ... Biophysical reviews 14 (6), 1281-1301, 2022 | 19 | 2022 |
Molecular interactions in the priming complex of bacteriophage T7 AW Kulczyk, CC Richardson Proceedings of the National Academy of Sciences 109 (24), 9408-9413, 2012 | 18 | 2012 |
Heterohexamer of 56-and 63-kDa gene 4 helicase-primase of bacteriophage T7 in DNA replication H Zhang, SJ Lee, AW Kulczyk, B Zhu, CC Richardson Journal of Biological Chemistry 287 (41), 34273-34287, 2012 | 17 | 2012 |
A crystal structure of coil 1B of vimentin in the filamentous form provides a model of a high‐order assembly of a vimentin filament AH Pang, JM Obiero, AW Kulczyk, VM Sviripa, OV Tsodikov The FEBS journal 285 (15), 2888-2899, 2018 | 16 | 2018 |
A robust single-particle cryo-electron microscopy (cryo-EM) processing workflow with cryoSPARC, RELION, and Scipion MC DiIorio, AW Kulczyk JoVE (Journal of Visualized Experiments), e63387, 2022 | 15 | 2022 |
Structural and functional probing of PorZ, an essential bacterial surface component of the type-IX secretion system of human oral-microbiomic Porphyromonas gingivalis. Sci Rep … AM Lasica, T Goulas, D Mizgalska, X Zhou, I De Diego, M Ksiazek, ... Front Cell Infect Microbiol 6 96, 2016 | 15 | 2016 |
Pyrovanadolysis, a pyrophosphorolysis-like reaction mediated by pyrovanadate, Mn2+, and DNA polymerase of bacteriophage T7 B Akabayov, AW Kulczyk, SR Akabayov, C Theile, LW McLaughlin, ... Journal of Biological Chemistry 286 (33), 29146-29157, 2011 | 10 | 2011 |
Cryo-EM structure of Shiga toxin 2 in complex with the native ribosomal P-stalk reveals residues involved in the binding interaction AW Kulczyk, COS Sorzano, P Grela, M Tchórzewski, NE Tumer, XP Li Journal of Biological Chemistry 299 (1), 2023 | 9 | 2023 |