The establishment of an apical–basal axis of polarity is essential for the organization and
functioning of epithelial cells. Polarization of epithelial cells is orchestrated by a network of …

To initiate studies on how protein-protein interaction (or “interactome”) networks relate to
multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans …

Plants generate effective responses to infection by recognizing both conserved and variable
pathogen-encoded molecules. Pathogens deploy virulence effector proteins into host cells, …

Systematic mapping of protein–protein interactions, or ‘interactome’ mapping, was initiated
in model organisms, starting with defined biological processes 1 , 2 and then expanding to …

To provide accurate biological hypotheses and elucidate global properties of cellular networks,
systematic identification of protein-protein interactions must meet high quality standards. …

Many protein-protein interactions are mediated through independently folding modular
domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks …

The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection
of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome …

An important aspect of the development of systems biology approaches in metazoans is the
characterization of expression patterns of nearly all genes predicted from genome …

Notch signaling is critical for cell fate decisions during development. Caenorhabditis elegans
and vertebrate Notch ligands are more diverse than classical Drosophila Notch ligands, …

Since its inception, the yeast two-hybrid (Y2H) system has proven to be an efficient system to
identify novel protein–protein interactions. However, Y2H screens are sometimes criticized …