Next-generation sequencing allows the analysis of genomes, including those representing
disease states. However, the causes of most disorders are multifactorial, and systems-level …

Native mass spectrometry is an emerging technology that allows the topological investigation
of intact protein complexes with high sensitivity and a theoretically unrestricted mass range…

Both the generation and the analysis of proteomics data are now widespread, and high-throughput
approaches are commonplace. Protocols continue to increase in complexity as …

Affinity purification coupled with mass spectrometry (AP-MS) is a widely used approach for
the identification of protein-protein interactions. However, for any given protein of interest, …

Accurate quantification of protein expression in biological systems is an increasingly important
part of proteomics research. Incorporation of differential stable isotopes in samples for …

The adult stem cell marker Lgr5 and its relative Lgr4 are often co-expressed in Wnt-driven
proliferative compartments. We find that conditional deletion of both genes in the mouse gut …

Selective detection of phosphopeptides from proteolytic digests is a challenging and highly
relevant task in many proteomics applications. Often phosphopeptides are present in small …

Degradation of cytosolic β-catenin by the APC/Axin1 destruction complex represents the
key regulated step of the Wnt pathway. It is incompletely understood how the Axin1 complex …

LGR5 + stem cells reside at crypt bottoms, intermingled with Paneth cells that provide Wnt,
Notch and epidermal growth factor signals 1 . Here we find that the related RNF43 and …

Two types of stem cells are currently defined in small intestinal crypts: cycling crypt base
columnar (CBC) cells and quiescent ‘+4’ cells. Here, we combine transcriptomics with …