Transformation In Escherichia coli: Studies On The Role Of The Heat Shock In Induction Of Competence
IM Van Die, HEN Bergmans… - Microbiology, 1983 - microbiologyresearch.org
Escherichia coli can be rendered competent for DNA uptake by exposure to a heat shock in
the presence of divalent cations. We have studied the influence of variations of the …
the presence of divalent cations. We have studied the influence of variations of the …
Genetic recombination in Escherichia coli: IV. Isolation and characterization of recombinaion-deficient mutants of Escherichia coli K12
PK Storm, WPM Hoekstra, PG De Haan… - … Molecular Mechanisms of …, 1971 - Elsevier
19 independent recombination-deficient mutants were isolated. 7 carried mutations that
mapped near or in the recB and recC genes between thyA and argA. 10 mutants carried …
mapped near or in the recB and recC genes between thyA and argA. 10 mutants carried …
Conjugation deficient E. coli K12 F- mutants with heptose-less lipopolysaccharide
LM Havekes, BJJ Lugtenberg… - Molecular and General …, 1976 - Springer
Two F - mutants deficient in conjugation with F-type donors are isolated and characterized.
Phenotypically, these mutants are similar; they have heptose-less lipopolysaccharide and …
Phenotypically, these mutants are similar; they have heptose-less lipopolysaccharide and …
Synthesis of Marasin and 9-Me-Marasin,(Nona-and Deca-6, 8-diyne-3, 4-dienol).
W de Graaf, A Smits, J Boersma, G van Koten… - Tetrahedron, 1988 - Elsevier
3,4-Pentadienol was protected with ethyl vinyl ether and regioselectively (70%) deprotonated
at the terminal position by treatment with BuLi in THF at low temperature. Starting from the …
at the terminal position by treatment with BuLi in THF at low temperature. Starting from the …
Expression of porcine pancreatic phospholipase A 2. Generation of active enzyme by sequence-specific cleavage of a hybrid protein from Escherichia coli
P Geus, CJ Bergh, O Kuipers, HM Verheij… - Nucleic acids …, 1987 - academic.oup.com
The cDNA coding for the porcine pancreatic prophospholipase A2 (proPLA) has been
cloned and expressed in E. coli . Expression of proPLA could only be obtained in the form of …
cloned and expressed in E. coli . Expression of proPLA could only be obtained in the form of …
Recombination in Escherichia coli III. Mapping by the gradient of transmission
PG De Haan, WPM Hoekstra, C Verhoef… - … Molecular Mechanisms of …, 1969 - Elsevier
Mapping of markers on the Escherichia coli chromosome by the gradient of transmission is
presented. The method appeared to be useful and accurate if chromosome withdrawal is …
presented. The method appeared to be useful and accurate if chromosome withdrawal is …
Exclusion in IncI-type Escherichia coli conjugations: the stage of conjugation at which exclusion operates
RA Hartskeerl, WPM Hoekstra - Antonie van Leeuwenhoek, 1984 - Springer
The stage at which exclusion operates in matings between donors belonging to the I-type
incompatibility group (IncI) was investigated. Mating between Escherichia coli cells harbouring …
incompatibility group (IncI) was investigated. Mating between Escherichia coli cells harbouring …
Degradation of Escherichia coli Chromosomal and Plasmid DNA in Serum
M Rozenberg-Arska, EC Salters… - …, 1984 - microbiologyresearch.org
Incubation of serum-sensitive [ 3 H]thymidine labelled Escherichia coli PC2166 (RSF1030)
and E. coli AM1281(pBR322) harbouring small plasmids (mol. wt 5·5 × 10 6 and 2·6 × 10 6 ) …
and E. coli AM1281(pBR322) harbouring small plasmids (mol. wt 5·5 × 10 6 and 2·6 × 10 6 ) …
Transformation in E. coli K12: Relation of linkage to distance between markers
WPM Hoekstra, PG De Haan, JEN Bergmans… - Molecular and General …, 1976 - Springer
E. coli K12 transformants, selected as leu + or pyrA + transformants, were analysed for
inheritance of some closely linked unselected markers. Based on the observation that the number …
inheritance of some closely linked unselected markers. Based on the observation that the number …
Cloning of an exclusion-determining fragment of the IncI plasmid, R144
RA Hartskeerl, JEN Bergmans, MC Kamp… - Plasmid, 1983 - Elsevier
By cloning a distinct 8 MDa fragment of the IncI plasmid, R144, in the vector pACYC184, two
recombinant plasmids were isolated. In these plasmids, pRAH303 and pRAH308, the …
recombinant plasmids were isolated. In these plasmids, pRAH303 and pRAH308, the …
