Progress with gene‐product mapping of the Mollicutes: Mycoplasma genitalium

VC Wasinger, SJ Cordwell, A Cerpa‐Poljak… - …, 1995 - Wiley Online Library
A protein map of the smallest known self‐replicating organism, Mycoplasma genitalium (Class:
Mollicutes), revealed a high proportion of acidic proteins. Amino acid composition was …

[PDF][PDF] Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it

MR Wilkins, JC Sanchez, AA Gooley… - Biotechnology and …, 1996 - Taylor & Francis
The advent of large genolne sequencing projects has changed the scale of biology. Over a
relatively short period of tinle, we have witnessed the elucidation of the conlplete nucleotide …

From proteins to proteomes: large scale protein identification by two-dimensional electrophoresis and arnino acid analysis

MR Wilkins, C Pasquali, RD Appel, K Ou, O Golaz… - Bio/technology, 1996 - nature.com
Separation and identification of proteins by two-dimensional (2-D) electrophoresis can be
used for protein-based gene expression analysis. In this report single protein spots, from …

Proteome research: complementarity and limitations with respect to the RNA and DNA worlds

I Humphery‐Smith, SJ Cordwell… - Electrophoresis, 1997 - Wiley Online Library
A methodological overview of proteome analysis is provided along with details of efforts to
achieve high‐throughput screening (HTS) of protein samples derived from two‐dimensional …

The evolution of phylogenetic specificity among parasitic organisms

I Humphery-Smith - Parasitology Today, 1989 - cell.com
It has long been recognized that, in some cases, the evolution ofparasites seems to follow
closely that of their hosts, yet in the literature dealing with host-parasite evolution, the causes …

Regionalized GC content of template DNA as a predictor of PCR success

Y Benita, RS Oosting, MC Lok, MJ Wise… - Nucleic acids …, 2003 - academic.oup.com
A set of 1438 human exons was subjected to nested PCR. The initial success rate using a
standard PCR protocol required for ligation‐independent cloning was 83.4%. Logistic …

Cross‐species identification of proteins separated by two‐dimensional gel electrophoresis using matrix‐assisted laser desorption ionisation/time‐of‐flight mass …

SJ Cordwell, MR Wilkins, A Cerpa‐Poljak… - …, 1995 - Wiley Online Library
Amino acid analysis and matrix‐assisted laser desorption/ionisation time‐of‐flight (MALDI‐TOF)
mass spectrometry were used to identify nine of twelve proteins originally separated by …

Pathogenicity of Spiroplasma taiwanense for larval Aedes aegypti mosquitoes

IAN HUMPHERY‐SMITH, O GRULET… - Medical and …, 1991 - Wiley Online Library
Helical replicative forms, but not the persistent non‐replicative forms, of Spiroplasma
taiwanense Abalain‐Colloc et al. (isolated from the mosquito Anopheles sinensis Wiedemann in …

Proteome analysis: genomics via the output rather than the input code

I Humphery-Smith, W Blackstock - Journal of protein chemistry, 1997 - Springer
A knowledge of the ‘proteome,’ total protein output encoded by a genome, provides information
on (1) if and when predicted gene products are translated, (2) the relative concentrations …

The proteome of Mycoplasma genitalium Chaps‐soluble component

VC Wasinger, JD Pollack… - European Journal of …, 2000 - Wiley Online Library
Mycoplasma genitalium is the smallest member of the class Mollicutes, with a genome size of
580 kb. It has the potential to express 480 gene products, and is therefore considered to be …