The evolutionary journey of Argonaute proteins
Argonaute proteins are conserved throughout all domains of life. Recently characterized
prokaryotic Argonaute proteins (pAgos) participate in host defense by DNA interference, …
prokaryotic Argonaute proteins (pAgos) participate in host defense by DNA interference, …
The genetics of RNA silencing
M Tijsterman, RF Ketting… - Annual Review of …, 2002 - annualreviews.org
▪ Abstract Although initially recognized as a handy tool to reduce gene expression, RNA
silencing, triggered by double-stranded RNA molecules, is now recognized as a mechanism for …
silencing, triggered by double-stranded RNA molecules, is now recognized as a mechanism for …
[PDF][PDF] The many faces of RNAi
RF Ketting - Developmental cell, 2011 - cell.com
Small non-coding RNAs, through association with Argonaute protein family members, have
a variety of functions during the development of an organism. Although there is increased …
a variety of functions during the development of an organism. Although there is increased …
Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans
RF Ketting, SEJ Fischer, E Bernstein… - Genes & …, 2001 - genesdev.cshlp.org
Double-stranded RNAs can suppress expression of homologous genes through an
evolutionarily conserved process named RNA interference (RNAi) or post-transcriptional gene …
evolutionarily conserved process named RNA interference (RNAi) or post-transcriptional gene …
Processing of primary microRNAs by the Microprocessor complex
Mature microRNAs (miRNAs) are generated via a two-step processing pathway to yield ∼22-nucleotide
small RNAs that regulate gene expression at the post-transcriptional level 1 . …
small RNAs that regulate gene expression at the post-transcriptional level 1 . …
[HTML][HTML] A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish
S Houwing, LM Kamminga, E Berezikov… - Cell, 2007 - cell.com
Piwi proteins specify an animal-specific subclass of the Argonaute family that, in vertebrates,
is specifically expressed in germ cells. We demonstrate that zebrafish Piwi (Ziwi) is …
is specifically expressed in germ cells. We demonstrate that zebrafish Piwi (Ziwi) is …
[HTML][HTML] Mut-7 of C. elegans, required for transposon silencing and RNA interference, is a homolog of Werner syndrome helicase and RNaseD
RF Ketting, THA Haverkamp, HGAM van Luenen… - Cell, 1999 - cell.com
While all known natural isolates of C. elegans contain multiple copies of the Tc1 transposon,
which are active in the soma, Tc1 transposition is fully silenced in the germline of many …
which are active in the soma, Tc1 transposition is fully silenced in the germline of many …
[HTML][HTML] Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development
WP Kloosterman, AK Lagendijk, RF Ketting… - PLoS …, 2007 - journals.plos.org
Several vertebrate microRNAs (miRNAs) have been implicated in cellular processes such as
muscle differentiation, synapse function, and insulin secretion. In addition, analysis of Dicer …
muscle differentiation, synapse function, and insulin secretion. In addition, analysis of Dicer …
A micrococcal nuclease homologue in RNAi effector complexes
RNA interference (RNAi) regulates gene expression by the cleavage of messenger RNA, by
mRNA degradation and by preventing protein synthesis. These effects are mediated by a …
mRNA degradation and by preventing protein synthesis. These effects are mediated by a …
Metabolic labeling of C. elegans and D. melanogaster for quantitative proteomics
J Krijgsveld, RF Ketting, T Mahmoudi, J Johansen… - Nature …, 2003 - nature.com
A crucial issue in comparative proteomics is the accurate quantification of differences in
protein expression levels. To achieve this, several methods have been developed in which …
protein expression levels. To achieve this, several methods have been developed in which …