Lysosomes are the primary catabolic compartments of eukaryotic cells. They degrade
extracellular material that has been internalized by endocytosis and intracellular components that …

Live-cell imaging reveals the endolysosomal system as a complex and highly dynamic
network of interacting compartments. Distinct types of endosomes are discerned by kinetic, …

Since its first visualization in 1898, the Golgi has been a topic of intense morphological research.
A typical mammalian Golgi consists of a pile of stapled cisternae, the Golgi stack, which …

The occurrence of clathrin-coated buds on immature granules (IGs) of the regulated secretory
pathway suggests that specific transmembrane proteins are sorted into these buds through …

Autophagy is an evolutionarily conserved process by which cytoplasmic proteins and organelles
are catabolized 1 , 2 . During starvation, the protein TOR (target of rapamycin), a nutrient…

A heterotetrameric complex termed AP-3 is involved in signal-mediated protein sorting to
endosomal-lysosomal organelles. AP-3 has been proposed to be a component of a nonclathrin …

The prevailing hypothesis is that the intracellular site of budding of coronaviruses is determined
by the localization of its membrane protein M (previously called E1). We tested this by …

We have determined the concentrations of the secretory proteins amylase and chymotrypsinogen
and the membrane proteins KDELr and rBet1 in COPII- and COPI-coated pre-Golgi …

The adaptor protein (AP) 3 adaptor complex has been implicated in the transport of lysosomal
membrane proteins, but its precise site of action has remained controversial. Here, we …

Transport from the endoplasmic reticulum (ER) to the Golgi complex requires assembly of the
COPII coat complex at ER exit sites. Recent studies have raised the question as to whether …