The poly-l-proline type II (PPII) helix in recent years has emerged clearly as a structural class
not only of fibrillar proteins (in collagen, PPII is a dominant conformation) but also of the …

Many ribonucleases (RNases) are highly cytotoxic. In some cases, they attack selectively
malignant cells, triggering apoptotic response, and therefore are considered as alternative …

Geroprotectors slow down aging and promote healthy longevity in model animals. Although
hundreds of compounds have been shown to extend the life of laboratory model organisms, …

The orbitrap mass analyzer employs the trapping of pulsed ion beams in an electrostatic
quadro-logarithmic field. This field is created between an axial central electrode and a coaxial …

The net charge and isoelectric pH (pI) of a protein depend on the content of ionizable groups
and their pK values. Ribonuclease Sa (RNase Sa) is an acidic protein with a pI = 3.5 that …

We evaluate the quantitative performance of the newly released Asymmetric Track Lossless
(Astral) analyzer. Using data-independent acquisition, the Thermo Scientific Orbitrap Astral …

DNA gyrase is a major bacterial protein that is involved in replication and transcription and
catalyzes the negative supercoiling of bacterial circular DNA. DNA gyrase is a known target …

The growing trend toward high-throughput proteomics demands rapid liquid
chromatography–mass spectrometry (LC–MS) cycles that limit the available time to gather the large …

Native mass spectrometry (MS) is becoming an important integral part of structural proteomics
and system biology research. The approach holds great promise for elucidating higher …

Na,K-ATPase is highly sensitive to changes in the redox state, and yet the mechanisms of
its redox sensitivity remain unclear. We have explored the possible involvement of S-…