Treatment of inflammatory bowel disease in childhood: best available evidence

JC Escher, JAJM Taminiau… - Inflammatory bowel …, 2003 - academic.oup.com
The physician treating children with inflammatory bowel disease is confronted with a number
of specific problems, one of them being the lack of randomized, controlled drug trials in …

[HTML][HTML] NKT cells in mucosal immunity

S Middendorp, EES Nieuwenhuis - Mucosal immunology, 2009 - Elsevier
The gastrointestinal tract allows the residence of an almost enumerable number of bacteria.
To maintain homeostasis, the mucosal immune system must remain tolerant to the …

Intestinal epithelial cell polarity defects in disease: lessons from microvillus inclusion disease

K Schneeberger, S Roth… - Disease models & …, 2018 - journals.biologists.com
The intestinal epithelium is a highly organized tissue. The establishment of epithelial cell
polarity, with distinct apical and basolateral plasma membrane domains, is pivotal for both …

[HTML][HTML] XBP1 links ER stress to intestinal inflammation and confers genetic risk for human inflammatory bowel disease

A Kaser, AH Lee, A Franke, JN Glickman, S Zeissig… - Cell, 2008 - cell.com
Inflammatory bowel disease (IBD) has been attributed to aberrant mucosal immunity to the
intestinal microbiota. The transcription factor XBP1, a key component of the endoplasmic …

[HTML][HTML] Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients

G Schwank, BK Koo, V Sasselli, JF Dekkers, I Heo… - Cell stem cell, 2013 - cell.com
Single murine and human intestinal stem cells can be expanded in culture over long time
periods as genetically and phenotypically stable epithelial organoids. Increased cAMP levels …

Long-term culture of genome-stable bipotent stem cells from adult human liver

M Huch, H Gehart, R Van Boxtel, K Hamer, F Blokzijl… - Cell, 2015 - cell.com
Despite the enormous replication potential of the human liver, there are currently no culture
systems available that sustain hepatocyte replication and/or function in vitro. We have shown …

A functional CFTR assay using primary cystic fibrosis intestinal organoids

JF Dekkers, CL Wiegerinck, HR De Jonge… - Nature medicine, 2013 - nature.com
We recently established conditions allowing for long-term expansion of epithelial organoids
from intestine, recapitulating essential features of the in vivo tissue architecture. Here we …

Tissue-specific mutation accumulation in human adult stem cells during life

F Blokzijl, J De Ligt, M Jager, V Sasselli, S Roerink… - Nature, 2016 - nature.com
The gradual accumulation of genetic mutations in human adult stem cells (ASCs) during life
is associated with various age-related diseases, including cancer 1 , 2 . Extreme variation in …

Characterizing responses to CFTR-modulating drugs using rectal organoids derived from subjects with cystic fibrosis

JF Dekkers, G Berkers, E Kruisselbrink… - Science translational …, 2016 - science.org
Identifying subjects with cystic fibrosis (CF) who may benefit from cystic fibrosis transmembrane
conductance regulator (CFTR)–modulating drugs is time-consuming, costly, and …

CD1d-dependent macrophage-mediated clearance of Pseudomonas aeruginosa from lung

EES Nieuwenhuis, T Matsumoto, M Exley… - Nature medicine, 2002 - nature.com
CD1d-restricted T cells are implicated as key players in host defense against various
microbial infections. However, the mechanisms involved and the role they play, if any, at the …