Purification and substrate specificity of Staphylococcus hyicus lipase

MG Van Oort, AMTJ Deveer, R Dijkman, ML Tjeenk… - Biochemistry, 1989 - ACS Publications
Revised Manuscript Received June 21, 1989 abstract: The Staphylococcus hyicus lipasegene
has been cloned and expressed in Staphylococcus carnosus. From the latter organism …

Structure and function of phospholiphase A2

HM Verheij, AJ Slotboom, GH De Haas - Reviews of Physiology …, 1981 - Springer
Phospholipases As (EC 3.1. 1.4) are wide spread in Nature and are found both within and
without the cell (see for reviews Shen and Law 1979; van den Bosch 1980). In this review we …

Staphylococcal lipases: molecular characterisation, secretion, and processing

F Götz, HM Verheij, R Rosenstein - Chemistry and physics of lipids, 1998 - Elsevier
Up to date five different staphylococcal lipase genes, two of Staphylococcus aureus (sal-1
and sal-2), two of Staphylococcus epidermidis (sel-1 and sel-2) and one of Staphylococcus …

Structural evidence for dimerization-regulated activation of an integral membrane phospholipase

HJ Snijder, I Ubarretxena-Belandia, M Blaauw, KH Kalk… - Nature, 1999 - nature.com
Dimerization is a biological regulatory mechanism employed by both soluble and membrane
proteins 1 . However, there are few structural data on the factors that govern dimerization of …

Methylation of histidine-48 in pancreatic phospholipase A2. Role of histidine and calcium ion in the catalytic mechanism

HM Verheij, JJ Volwerk, E Jansen, WC Puyk… - Biochemistry, 1980 - ACS Publications
A2, we have modified the enzyme with a number of bromo ketones and methyl
benzenesulfonates. Rapid methylation occurred with methyl p-nitrobenzenesulfonate. Methylated …

A monolayer and bulk study on the kinetic behavior of Pseudomonas glumae lipase using synthetic pseudoglycerides

AMTJ Deveer, R Dijkman, M Leuveling-Tjeenk… - Biochemistry, 1991 - ACS Publications
Department for Enzymology and Protein Engineering, CBLE, Trans III, Padualaan 8, 3584 CH
Utrecht, The Netherlands, Centre de Biochimie et Biologie Moleculaire, CNRS, 31 Chemin …

Biochemical properties of staphylococcal (phospho) lipases

JWFA Simons, F Götz, MR Egmond… - Chemistry and physics of …, 1998 - Elsevier
Various staphylococci secrete lipases which require calcium ions for activity, and have profound
preferences for substrates with different chain lengths. The lipase from Staphylococcus …

Structural basis of the chiral selectivity of Pseudomonas cepacia lipase

DA Lang, MLM Mannesse, GH De Haas… - European journal of …, 1998 - Wiley Online Library
To investigate the enantioselectivity of Pseudomonas cepacia lipase, inhibition studies
were performed with S C ‐ and R C ‐(R P ,S P )‐1,2‐dialkylcarbamoylglycero‐3‐O‐p‐nitrophenyl …

Correlation of Enzymatic Activity and Anticoagulant Properties of Phospholipase A2

HM Verheij, MC BOFFA, C Rothen… - European journal of …, 1980 - Wiley Online Library
Some highly purified phospholipases A from the venom of viperidae, crotalidae and
elapidae were found to have anticoagulant properties. All phospholipases which exhibited …

X-ray structure of phospholipase A2 complexed with a substrate-derived inhibitor

MMGM Thunnissen, E Ab, KH Kalk, J Drenth… - Nature, 1990 - nature.com
PHOSPHOLIPASES A 2 play a part in a number of physiologically important cellular processes
such as inflammation, blood platelet aggregation and acute hypersensitivity 1,2 . These …