The role of ammonia metabolism in nitrogen catabolite repression in Saccharomyces cerevisiae

EG ter Schure, NAW van Riel… - FEMS microbiology …, 2000 - academic.oup.com
Saccharomyces cerevisiae is able to use a wide variety of nitrogen sources for growth. Not
all nitrogen sources support growth equally well. In order to select the best out of a large …

Immobilizing proteins on the surface of yeast cells

MP Schreuder, ATA Mooren, HY Toschka… - Trends in …, 1996 - cell.com
Yeast has a rigid cell wall comprising an outer layer of glycoproteins and an internal
skeletal layer of glucan; heterologous proteins can be targeted to the glycoprotein layer and …

Nanobodies that neutralize HIV

RA Weiss, CT Verrips - Vaccines, 2019 - mdpi.com
Nanobodies or VHH (variable domains of heavy-chain only antibodies) are derived from
camelid species such as llamas and camels. Nanobodies isolated and selected through phage …

An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains

JP Van Dijken, J Bauer, L Brambilla, P Duboc… - Enzyme and microbial …, 2000 - Elsevier
To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques
used in (molecular) genetic, physiological and biochemical engineering research, a …

Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies

RHJ Van der Linden, LGJ Frenken, B De Geus… - … et Biophysica Acta (BBA …, 1999 - Elsevier
Antigen specific llama V HH antibody fragments were compared to antigen specific mouse
monoclonal antibodies with respect to specificity, affinity and stability. The llama V HH …

Isolation of antigen specific llama VHH antibody fragments and their high level secretion by Saccharomyces cerevisiae

LGJ Frenken, RHJ Van Der Linden… - Journal of …, 2000 - Elsevier
Recently the existence of ‘heavy chain’ immunoglobulins in Camelidae has been described.
However, as yet there is no data on the binding of this type of antibody to haptens. In …

Glycosylation profiling of immunoglobulin G (IgG) subclasses from human serum

M Wuhrer, JC Stam, FE van de Geijn… - …, 2007 - Wiley Online Library
All four subclasses of human serum IgG contain a single N‐glycosylation site in the constant
region of their heavy chain, which is occupied by biantennary, largely core‐fucosylated and …

Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae

JM van der Vaart, LH Caro, JW Chapman… - Journal of …, 1995 - Am Soc Microbiol
Three glucanase-extractable cell wall proteins from Saccharomyces cerevisiae were purified,
and their N-terminal amino acid sequences were determined. With this information, we …

Cloning of cDNA encoding the sweet-tasting plant protein thaumatin and its expression in Escherichia coli

L Edens, L Heslinga, R Klok, AM Ledeboer, J Maat… - Gene, 1982 - Elsevier
The structural gene of the sweet-tasting plant protein (prepro) thaumatin was cloned and
expressed in Escherichia colt. Expression was effected under control of lac and trp promoter/…

Transformation of Aspergillus awamori by Agrobacterium tumefaciens–mediated homologous recombination

RJ Gouka, C Gerk, PJJ Hooykaas, P Bundock… - Nature …, 1999 - nature.com
Agrobacterium tumefaciens is known to transfer part of its tumor-inducing (Ti) plasmid to the
filamentous fungus Aspergillus awamori by illegitimate recombination with the fungal …